Resume of Mu Cheng

Contact Information

Name: Mu CHENG

Address: 70/139 Macquarie St, St Lucia, QLD 4067

or

Career Objective

To gain a position as a research assistant in biotechnology or any relevant field and to gain further experience in this area.

Education

Master of Biotechnology (Advanced) ︱ Feb 2007 to Nov 2008

The University of Queensland ︱ Australia

• Major field: environmental microbiology

• Overall GPA: credit average (5.75/7.0)

• Master Thesis: Extraction and characterization of extracellular

DNA (e-DNA) produced by aerobic granules in activated sludge

• Research project: I was responsible for extracting extracellular

DNA from aerobic granular sludge treated with abattoir wastewater using the cation exchange resin extraction method. I was also responsible for comparing the composition of extracellular DNA and cellular DNA through several molecular-based techniques such as by examination of randomly amplified polymorphic DNA (RAPD) on agarose gel and by analysis of 16S rRNA gene after PCR amplification and separation by denaturing gradient gel electrophoresis (DGGE).

Bachelor of Biological Education ︱ Sep 2002 to Jul 2006

The Nanjing Normal University ︱ Australia

• Major field: biological education

• Overall GPA: 83%

• Graduate Training: high school teacher in Nanjing 29 High School

Professional Experience

Lab Assistant ︱ Dec 2008 to Present

Advanced Water Management Center, the University of Queensland ︱ Australia

• Used software — GelCompar II to analyze banding patterns of DGGE gel

• Performed experiments consisting of subsequent PCR of excised DGGE gel bands and purification of PCR products using the QIAGEN QIAquick PCR Purification Kit

• Used the ChromasPro1.41 software to analyze DNA sequences and used BLAST to identify extracellular DNA sequences

• Assisted to Dr. Yang Mu with examination of microbe producing nitro-aromatic compounds in the microbial fuel cells (MFC) and bio-electrochemical systems.

Technical Skills

• PCR assay

• DNA and protein extraction from bacteria

• DNA, protein and carbohydrate quantification assay

• Cloning & establishing clone library

• Randomly amplified polymorphic DNA (RAPD) fingerprinting

• Denaturing gradient gel electrophoresis (DGGE)

• Cell culture and transformation

• Protein purification and characterization including liquid chromatography and SDS-PAGE

• Media preparation

• Bioinformatics (i.e. BLAST, PDB, SWISS-PROT, GenBank)

• microscope handling and use

• FISH (Fluorescence in situ hybridization)

Computer Skills

• Have knowledge of MS Word, MS Access, MS Excel, MS Power Point and Photoshop

Abilities

• Effective oral communication and English written skill

• Can carry out in-depth research to bring out new findings

• Ability to work independently and as a cohesive team member

• Fast learner and ability to grasp new information quickly

• Meticulous in work approach

• Well organized

• Confident about completing the work on time

References

Dr Phil Bond

Project supervisor

Advanced Water Management Center, Australia

Ph: +61 (0)7 3446 7841

Dr Toshikazu Fukushima

Project co-supervisor

Department of Environmental Engineering, National Cheng Kung University, Taiwan

Dr Andrew Cook

Project co-supervisor

Advanced Water Management Center, Australia

Ph: +61 (0)7 3365 4645

Dr Yang Mu

MFC project supervisor

Advanced Water Management Center, Australia

Ph: +61 (0)7 3346 7211

Prof Ross Barnard

Master Course Coordinator

The University of Queensland, Australia

Ph: +61 (0)7 3365 4612

Declaration

I announce that the above mentioned information given by me is accurate and true according to my knowledge.