RESUME
NAME : Ms. PRAJAKTA RAMDAS AKOTKAR
SPECIALIZATION : M.Sc. -BIOTECHNOLOGY
PERSONAL DETAILS:
Correspondence Address : E/94/6, Koradi colony
District-Nagpur,
Maharashtra -441111
Contact
Date of Birth : 10th May 1983
Nationality : Indian
Marital status : Single
Languages known : Marathi, English & Hindi
OBJECTIVE :
Seeking an opportunity where my knowledge can be shared & enriched, as well as to have an opportunity to contribute effectively towards the growth of an organization.
PROFESSIONAL QUALIFICATION:
Passed M.Sc Biotechnology from RTM Nagpur University with 1st DIV (60.7%) in the year 2006.
EDUCATIONAL QUALIFICATION:
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Passed 12th std. from Maharashtra state board with 1st DIV. (76.17%) in the year 2001.
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Passed B.Sc (Biotechnology, Zoology, Chemistry) from RTM Nagpur University with 1st DIV (66.23%) in the year 2004.
WORK EXPERIENCE:
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Worked on “Agrobacterium mediated transformation of Gossyipium hirusitum with Bt genes through shoot tip culture” at National Research Centre for Cotton, Nagpur during the period Feb to April 2007.
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Worked as Jr. Research Fellow at National Research Centre for Citrus under the Project “ Large scale production and demonstration of virus free planting material through micrografting in Citrus reticulata cv. Nagpur mandarin” since May 2007 to Feb 2009.
ON JOB TRAINING UNDERTAKEN AT GRADUATION:
1) COMPANY NAME : RAINBOW MEDINOVA DIAGNOSTIC SERVICES,
NAGPUR.
TITLE : Training on Biophysics.
DURATION : 8 days.
SYNOPSIS : Western-Blotting, ELISA, ECG, EEG, CAT-SCAN,
Color doppler, ultrasound, Pathology lab tests, Operation
of blood bank, Nuclear medicine administration & tracing
Techniques.
2) COMPANY NAME : CENTRAL INSTITUTE OF COTTON RESEARCH (NAGPUR).
TITLE : Training on “Molecular Biology and Plant Tissue Culture.
DURATION : 10days.
SYNOPSIS : Training on Protoplast fusion, Isolation of DNA,
Southern Blotting, PCR, Restriction and Ligation
of DNA, Electroelution, Agrobacterium mediated
transformation , Particle Gun Bombardment method of
transformation.
PROJECTS DONE (As a Part of Curriculum):
1) TITLE : Molecular & Biochemical characterization of genomic DNA from some members of family Nyctageniaceae
PLACE OF WORK : L.A.D college, Nagpur.
2) TITLE : Enzymatic & Genomic Characterization of Microbial flora
isolated from tea waste
PLACE OF Sindhu Mahavidyalaya, Nagpur.
TECHNIQUES KNOWN:
Molecular techniques- DNA isolation (genomic & plasmid), Agarose gel electrophoresis, Polyacrylamide gel electrophoresis, Southern blotting, PCR.
Biochemical techniques- Protein, DNA& RNA estimation, enzyme activity determination.
Microbiological techniques- Media preparation & sterilization , pure culturing staining & identification of a microorganism, Antibiotic sensitivity, RBC & WBC count by haemocytometer, cell size determination.
Immunological techniques- Radial immunodiffusion, Oucterlony double immunodiffusion, ELISA.
Plant tissue culture techniques- Media preparation & sterilization, multiple shoot induction, initiation & maintenance of callus & suspension culture, shoot tip culture, protoplast isolation.
Animal tissue culture techniques- Media preparation & sterilization, establishment & maintenance of cell lines.
Computer skills:
Operating Systems : Windows 98, XP
Software Packages : MS WORD, MS EXCEL, MS POWERPOINT
Participation:
1)‘BIOTALK 2005’ on Cancer
2) Seminar held on topic “AN APPROACH FROM ACADEMICS TO INDUSTRY VIA SYNERGY”
3) Participated in Poster Presentation Competition held at National Symposium on Citriculture: Emerging trends organized by NRC for Citrus, Nagpur from 23-25th July 2008.
Undertaking
I, Prajakta Akotkar hereby declare that all the above information furnished by me is true to the best of my knowledge.
PRAJAKTA R. AKOTKAR
