Objective:

Quest to work hard and learn through experience with the scope of widening the spectrum of my knowledge and skills

Educational qualifications:

Master of Philosophy in Microbiology, C.C.S.U, India, 2005-2006, First division

Master of Science in Biotechnology, H.N.B.G.U, India, 2003-2006, First division

Bachelor of Science, H.N.B.G.U, India, 2000-2003, First division

Research Experience:

• C.C.S University India, 2006 to 2008

Worked on Project “ A Study on Heavy Metal Resistant Bacteria Isolated from Roots of Eichhornia crassipes”

The increasing amount of heavy metals poses a potential hazard to the ecosystem. Many microbes are able to regulate metal concentration in living tissues. This process plays an important role in future of industrial waste management. In the study, the bacteria capable of selected resistance against various heavy metals like Arsenic oxide, Nickel oxide was investigated. These bacteria were isolated from the roots of Eichhornia crassipes, which were collected from heavy metal polluted site. The bacteria were isolated under varying concentration of heavy metals and various biochemical tests were performed to identify them. The four bacteria isolated were Pseudomonas aeruginosa, Micrococcus luteus, Bacillus subtilis and Proteus vulgaris. The bacterial colony growth at different concentration of heavy metals was studied. The comparison of all the metals and growth pattern of the bacteria showed that most toxic metal out of five was Arsenic oxide and least toxic was Copper oxide. The most resistant bacteria out of four was Pseudomonas aeruginosa and the least resistant was Proteus vulgaris.

• Indian Institute of Petroleum 2005

Worked on Project “Biological Production of Hydrogen and Biodegradation of Petroleum Product”

Bioremediation technologies can accelerate the natural process to degrade oily wastes as cheaper alternative to expensive remediation processes like incineration and landfill. The analysis of biodegradability of petroleum waste product was evaluated using petroleum sludge wastes. Enzymes produced by microorganisms in the presence of carbon sources results in breakdown of the hydrocarbon molecule. Eighteen strains of bacteria were isolated from activated sludge purifying petroleum-refining wastewaters. These strains were plated on solidified mineral medium supplemented with oil fraction. Four of the strains that grew best in the presence of oil were selected for further studies. The strains were identified based on Bondes’ scheme and microscopic observation. Three of these belonged to the genus Arthobacter and one to the genus Micrococcus. Stationary cultures of single strain and mixtures of these were set up in mineral medium containing oil as sole carbon source. The breakdown of oil waste was found to be most effective by mixture of the strains than single strain

Work Experience:

Worked as a Lecturer Microbiology and Environmental Science at D.B.I.T Dehradun from Feb’07 to Nov’07

Techniques Acquainted:

Microscopy, Sterilization, Autoclave, Microbial culture, Media preparation, Aseptic transfer, Colony count, Staining techniques, Biochemical tests, Anaerobic microbial culture

Spectrophotometry, Centrifugation, Electrophoresis, Chromatography

DNA isolation, RNA isolation, SDS-PAGE Gel electrophoresis, PCR

ELISA, Western blotting

Relevant Coursework:

Microbiological Techniques

Microbial Technology

Biological and Radiotracer techniques

Membrane Biophysics, Cell and Developmental Biology

Microbiology, Immunology and Immunological techniques

RDT and Genomics

Enzymology and Protein Engineering

Animal and Plant tissue culture

Environmental Biotechnology and Bioprocess Engineering

Relevant Test Scores:

• GRE : Verbal 690, Quantitative 630

• TOEFL : 102

Computer Skills:

Windows 2000/XP, Microsoft Word, Microsoft Excel, Microsoft PowerPoint, Microsoft Outlook

References:

Professor P.K. Sharma

Head and Co-ordinator Department of Microbiology

C.C.S. University, Meerut

Phone 0-121-2600909

Email: pks264@rediffmail.com

Professor Asha Chandola Saklani

Head and Co-ordinator Department of Biotechnology

H.N.B Gharhwal University, Srinagar

Phone 01370-267427

Email: ashachandola@yahoo.co.in